It has been known that glutathione S-transferase π(GST-π) is overexpressed in chemically induced precancerous or cancerous lesion of the hepatocyte in animal models. Unlike most results of animal models, however, there has been a controvery
concerning
abut the application of GSTπto human liver cancer as a tumor marker. So this study was carried out to evaluate the expression and the possibility of GST-π as an immunohistochemical marker in hepatocellular carcinoma and cholangiocarcinoma.
Labeled streptavidin biotin method after heat pretreatment with microwave oven was performed in formalin-fixed, paraffin-embedded tissue taken from wedged or needle biopsied 16 cases of normal liver tissue, 20 hepatocellular carcinoma and 20
cholangiocarcinoma, respectively. The overexpression of GST-π was found in only three(15%) of 20 cases of hepatocellular carcinoma, but in sixteen(80%) of 20 cases of cholangiocarcinoma. The expression of GST-π in hepatocellular carcinoma
showed
a
tendency to be increased with lower histologic grade, but that in cholangiocarcinoma revealed tendency to be decreased with lower histologic grade. In conclusion, the results suggested that the expression of GST-π would be and
immunohistochemical
marker of human cholangiocarcinoma, rather than hepatocellular carcinoma unlike the results of GST-π expression during chemically induced animal hepatocarcinogenesis.
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